Petri_dish

 Petri_dish

is a shallow transparent lidded dish that biologists use to hold growth medium in which cells can be cultured, originally, cells of bacteria, fungi and small mosses. The container is named after its inventor, German bacteriologist Julius Richard Petri. It is the most common type of culture plate.

 

Place the Petri dishes in a warm, dark place.

 

If necessary, you can place the Petri dishes in a cooler location, but the bacteria will grow a lot more slowly. Leave the bacteria to develop for 4-6 days, as this will give the cultures enough time to grow.

Most bacteria collected in your environment will not be harmful. However, once they multiply into millions of colonies in a Petri dish they become more of a hazard. Be sure to protect open cuts with rubber gloves and never ingest or breathe in growing bacteria.

 

Procedure for making Petri dishes.

Measure the recommended amount of agar and distilled water in to a clean, sterile flask or beaker.

Using heat resistant hand protection, hold the beaker/flask over a flame and stir the mixture gently using a sterile stir rod while heating.

Continue boiling the mixture for about one minute, and then remove from heat.

 

Pour about 12 ml into each Petri dish. If too little agar is poured, there may not be enough to cover the dish or the agar plate will dry up easily. If too much is poured, the cover dish will come in contact with the nutrient agar, leaving no room for microbial growth. The plates are rendered useless either way.



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